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Evaluating nephrotoxicity of high-molecular-weight organic compounds in drinking water from lignite aquifers

Journal of Toxicology and Environmental Health, Part A

By:
, , , , and
DOI: 10.1080/15287390701551274

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Abstract

High-molecular-weight organic compounds such as humic acids and/or fulvic acids that are naturally mobilized from lignite beds into untreated drinking-water supplies were suggested as one possible cause of Balkan endemic nephropathy (BEN) and cancer of the renal pelvis. A lab investigation was undertaken in order to assess the nephrotoxic potential of such organic compounds using an in vitro tissue culture model. Because of the infeasibility of exposing kidney tissue to low concentrations of organics for years in the lab, tangential flow ultrafiltration was employed to hyperconcentrate samples suitable for discerning effects in the short time frames necessitated by tissue culture systems. Effects on HK-2 kidney cells were measured using two different cell proliferation assays (MTT and alamarBlue). Results demonstrated that exposure of kidney tissue to high-molecular-weight organics produced excess cell death or proliferation depending on concentration and duration of exposure. Copyright ?? Taylor & Francis Group, LLC.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Evaluating nephrotoxicity of high-molecular-weight organic compounds in drinking water from lignite aquifers
Series title:
Journal of Toxicology and Environmental Health, Part A
DOI:
10.1080/15287390701551274
Volume
70
Issue:
24
Year Published:
2007
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of Toxicology and Environmental Health, Part A
First page:
2089
Last page:
2091
Number of Pages:
3