Cross-kingdom amplification using Bacteria-specific primers: Complications for studies of coral microbial ecology
Applied and Environmental Microbiology
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Abstract
PCR amplification of pure bacterial DNA is vital to the study of bacterial interactions with corals. Commonly used Bacteria-specific primers 8F and 27F paired with the universal primer 1492R amplify both eukaryotic and prokaryotic rRNA genes. An alternative primer set, 63F/1542R, is suggested to resolve this problem.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Cross-kingdom amplification using Bacteria-specific primers: Complications for studies of coral microbial ecology |
| Series title | Applied and Environmental Microbiology |
| DOI | 10.1128/AEM.01303-08 |
| Volume | 74 |
| Issue | 24 |
| Year Published | 2008 |
| Language | English |
| Publisher | ASM journals |
| Contributing office(s) | St. Petersburg Coastal and Marine Science Center |
| Description | 4 p. |
| First page | 7828 |
| Last page | 7831 |
| Google Analytic Metrics | Metrics page |