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Molecular method for determining sex of walruses

Journal of Wildlife Management

By:
, , , , , and
DOI: 10.2193/2007-413

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Abstract

We evaluated the ability of a set of published trans-species molecular sexing primers and a set of walrus-specific primers, which we developed, to accurately identify sex of 235 Pacific walruses (Odobenus rosmarus divergens). The trans-species primers were developed for mammals and targeted the X- and Y-gametologs of the zinc finger protein genes (ZFX, ZFY). We extended this method by using these primers to obtain sequence from Pacific and Atlantic walrus (0. r. rosmarus) ZFX and ZFY genes to develop new walrus-specific primers, which yield polymerase chain reaction products of distinct lengths (327 and 288 base pairs from the X- and Y-chromosome, respectively), allowing them to be used for sex determination. Both methods yielded a determination of sex in all but 1-2% of samples with an accuracy of 99.6-100%. Our walrus-specific primers offer the advantage of small fragment size and facile application to automated electrophoresis and visualization.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Molecular method for determining sex of walruses
Series title:
Journal of Wildlife Management
DOI:
10.2193/2007-413
Volume
72
Issue:
8
Year Published:
2008
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of Wildlife Management
First page:
1808
Last page:
1812