| Abstract: | Aims: The genetic relationships and conidial tolerances to high and low temperatures were determined for isolates of several Metarhizium species and varieties. Methods and Results: Molecular-based techniques [AFLP and rDNA (ITS1, ITS2 and 5??8S) gene sequencing] were used to characterize morphologically identified Metarhizium spp. isolates from a wide range of sources. Conidial suspensions of isolates were exposed to wet heat (45 ?? 0??2??C) and plated on potato dextrose agar plus yeast extract (PDAY) medium. After 8-h exposure, the isolates divided clearly into two groups: (i) all isolates of Metarhizium anisopliae var. anisopliae (Ma-an) and Metarhizium from the flavoviride complex (Mf) had virtually zero conidial relative germination (RG), (ii) Metarhizium anisopliae var. acridum (Ma-ac) isolates demonstrated high heat tolerance (c. 70-100% RG). Conidial suspensions also were plated on PDAY and incubated at 5??C for 15 days, during which time RGs for Ma-an and Ma-ac isolates were virtually zero, whereas the two Mf were highly cold active (100% RG). Conclusions: Heat and cold exposures can be used as rapid tools to tentatively identify some important Metarhizium species and varieties. Significance and Impact of the Study: Identification of Metarhizium spp. currently relies primarily on DNA-based methods; we suggest a simple temperature-based screen to quickly obtain tentative identification of isolates as to species or species complexes. ?? 2009 The Society for Applied Microbiology. |
| Genre: | Article |
| ProdID: | 70033808 |
| Citation Author: | Fernandes, E. K. K.; Keyser, C. A.; Chong, J. P.; Rangel, D. E. N.; Miller, M. P.; Roberts, D. W. |
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| Citation End Page: | 128 |
| Citation Issue: | 1 |
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| Citation Language: | English |
| Citation Larger Work Title: | Journal of Applied Microbiology |
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| Citation Number Of Pages: | 14 |
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| Citation Search Results Text: | Characterization of Metarhizium species and varieties based on molecular analysis, heat tolerance and cold activity; 2010; Article; Journal; Journal of Applied Microbiology; Fernandes, E. K. K.; Keyser, C. A.; Chong, J. P.; Rangel, D. E. N.; Miller, M. P.; Roberts, D. W. |
| Citation Start Page: | 115 |
| Citation Volume: | 108 |
| Citation Year: | 2010 |
| Type: | citation/reference |
| Text: | Characterization of Metarhizium species and varieties based on molecular analysis, heat tolerance and cold activity; 2010; Article; Journal; Journal of Applied Microbiology; Fernandes, E. K. K.; Keyser, C. A.; Chong, J. P.; Rangel, D. E. N.; Miller, M. P.; Roberts, D. W. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| URL (DIGITAL OBJECT IDENTIFIER): | http://dx.doi.org/10.1111/j.1365-2672.2009.04422.x |
| Date Other: | Fri, 1 Jan 2010 00:00 -0600 |
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