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Development of a molecular diagnostic system to discriminate Dreissena polymorpha (zebra mussel) and Dreissena bugensis (quagga mussel)

Molecular Ecology Resources

By:
, , and
DOI: 10.1111/j.1755-0998.2009.02727.x

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Abstract

A 3-primer PCR system was developed to discriminate invasive zebra (Dreissena polymorpha) and quagga (Dreissena bugensis) mussel. The system is based on: 1) universal primers that amplifies a region of the nuclear 28s rDNA gene from both species and 2) a species-specific primer complementary to either zebra or quagga mussel. The species-specific primers bind to sequences between the binding sites for the universal primers resulting in the amplification of two products from the target species and one product from the nontarget species. Therefore, nontarget products are positive amplification controls. The 3-primer system accurately discriminated zebra and quagga mussels from seven geographically distinct populations.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Development of a molecular diagnostic system to discriminate Dreissena polymorpha (zebra mussel) and Dreissena bugensis (quagga mussel)
Series title:
Molecular Ecology Resources
DOI:
10.1111/j.1755-0998.2009.02727.x
Volume
10
Issue:
1
Year Published:
2010
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Molecular Ecology Resources
First page:
190
Last page:
192
Number of Pages:
3