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Rapid polymerase chain reaction diagnosis of white-nose syndrome in bats

Journal of Veterinary Diagnostic Investigation

By:
, , , , , , , and

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Abstract

A newly developed polymerase chain reaction (PCR)-based method to rapidly and specifically detect Geomyces destructans on the wings of infected bats from small quantities (1-2 mg) of tissue is described in the current study (methods for culturing and isolating G. destructans from bat skin are also described). The lower limits of detection for PCR were 5 fg of purified fungal DNA or 100 conidia per 2 mg of wing tissue. By using histology as the standard, the PCR had a diagnostic specificity of 100% and a diagnostic sensitivity of 96%, whereas the diagnostic sensitivity of culture techniques was only 54%. The accuracy and fast turnaround time of PCR provides field biologists with valuable information on infection status more rapidly than traditional methods, and the small amount of tissue required for the test would allow diagnosis of white-nose syndrome in live animals.

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Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Rapid polymerase chain reaction diagnosis of white-nose syndrome in bats
Series title:
Journal of Veterinary Diagnostic Investigation
Volume
22
Issue:
2
Year Published:
2010
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of Veterinary Diagnostic Investigation
First page:
224
Last page:
230
Country:
United States
State:
Alabama, Connecticut, Indiana, Maryland, New Hampshire, New Jersey, New York, Ohio, Pennsylvania, Virginia, Vermont, West Virginia, Wisconsin
Online Only (Y/N):
N
Additional Online Files(Y/N):
N