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Attempts to identify Clostridium botulinum toxin in milk from three experimentally intoxicated Holstein cows

Journal of Dairy Science

By:
, , , , , , and
DOI: 10.3168/jds.2008-1919

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Abstract

Three adult lactating Holstein cows were injected in the subcutaneous abdominal vein with 175 ng/kg of body weight of Clostridium botulinum type C toxin (451 cow median toxic doses) to determine if this botulinum toxin crosses the blood-milk barrier. Whole blood (in sodium heparin) and clotted blood serum samples were taken at 0 min, 10 min, and 3, 6, 9, and 12 h postinoculation. Milk samples were taken at 0 min and at 3, 6, 9 and 12 h postinoculation. All samples were tested for the presence of the toxin using the mouse bioassay and immunostick ELISA test. The immunostick ELISA identified the toxin in whole blood and the mouse bioassay identified the toxin in serum at all times examined in all 3 animals. Toxin was not identified by either detection method in milk samples collected from the 3 animals. From these results, it appears that Clostridium botulinum type C toxin does not cross from the blood to the milk in detectable concentrations. ?? American Dairy Science Association, 2009.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Attempts to identify Clostridium botulinum toxin in milk from three experimentally intoxicated Holstein cows
Series title:
Journal of Dairy Science
DOI:
10.3168/jds.2008-1919
Volume
92
Issue:
6
Year Published:
2009
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of Dairy Science
First page:
2529
Last page:
2533
Number of Pages:
5