Two new ant-nest trace fossils are described from calcic sandy paleosols of the Neogene Ogallala Formation in western Kansas. The ichnofossils are preserved within and below calcrete beds weathering in positive relief as carbonate-filled casts or as cavities in negative relief. Daimoniobarax ichnogenus nov. is established for burrow systems composed of vertically tiered, horizontally oriented pancake-shaped chambers connected by predominantly vertical and cylindrical shafts ~ 0.8 cm in diameter. Ichnospecies of Daimoniobarax are differentiated based on differences in the plan view outline of chambers, shaft orientation, and junctions between chambers and shafts.

Daimoniobarax nephroides ichnospecies nov. is composed of an ~ 24–76 cm long vertical sequence of distinctly lobed chambers (~ 2–20 cm wide and ~ 1 cm high) arranged along sinuous to helical shafts. Chamber shape in plan view ranges from small teardrops to larger kidney- and U-shaped forms. Shafts intersect at chamber edges such that chambers appear to bud from the central shafts. Daimoniobarax nephroides is most similar to the nests of extant seed-harvester ants of the New World genus Pogonomyrmex. Such ants are specialized granivores and prefer sandy soils in arid to semi-arid grassland and desert regions.

Daimoniobarax tschinkeli ichnospecies nov. is ~ 30–80 cm in vertical extent. Chambers (~ 2–30 cm wide and ~ 1 cm high) are circular to elongate or pseudopodial in plan view. Vertical shafts are straight to slightly sinuous and intersect most often toward the center of the chambers. The generalized architecture of D. tschinkeli is similar to that of the nests or nest portions of several extant ant genera, though it does not closely resemble any known modern nest.

Ant ichnofossils provide valuable information on hidden biodiversity, paleohydrologic regimes, paleopedogenic processes, and paleoclimate during the time of nest occupation. Depth-related changes in chamber size and vertical spacing may also help interpret paleosurfaces and paleodepths, and serve as geopetal features.