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Identification of CD3+ T lymphocytes in the green turtle Chelonia mydas

Veterinary Immunology and Immunopathology

By:
, , , , , and
DOI: 10.1016/j.vetimm.2009.04.015

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Abstract

To understand the role of the immune system with respect to disease in reptiles, there is the need to develop tools to assess the host's immune response. An important tool is the development of molecular markers to identify immune cells, and these are limited for reptiles. We developed a technique for the cryopreservation of peripheral blood mononuclear cells and showed that a commercially available anti-CD3 epsilon chain antibody detects a subpopulation of CD3 positive peripheral blood lymphocytes in the marine turtle Chelonia mydas. In the thymus and in skin inoculated with phytohemagglutinin, the same antibody showed the classical staining pattern observed in mammals and birds. For Western blot, the anti-CD3 antibodies identified a 17.6 kDa band in membrane proteins of peripheral blood mononuclear cell compatible in weight to previously described CD3 molecules. This is the first demostration of CD3+ cells in reptiles using specific antibodies. ?? 2009 Elsevier B.V.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Identification of CD3+ T lymphocytes in the green turtle Chelonia mydas
Series title:
Veterinary Immunology and Immunopathology
DOI:
10.1016/j.vetimm.2009.04.015
Volume
131
Issue:
3-4
Year Published:
2009
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Veterinary Immunology and Immunopathology
First page:
211
Last page:
217