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Characterization of recombinant Raccoonpox Vaccine Vectors in Chickens

Avian Diseases

By:
, , , ,
DOI: 10.1637/9315-032410-Reg.1

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Abstract

Raccoonpox virus (RCN) has been used as a recombinant vector against several mammalian pathogens but has not been tested in birds. The replication of RCN in chick embryo fibroblasts (CEFs) and chickens was studied with the use of highly pathogenic avian influenza virus H5N1 hemagglutinin (HA) as a model antigen and luciferase (luc) as a reporter gene. Although RCN replicated to low levels in CEFs, it efficiently expressed recombinant proteins and, in vivo, elicited anti-HA immunoglobulin yolk (IgY) antibody responses comparable to inactivated influenza virus. Biophotonic in vivo imaging of 1-wk-old chicks with RCN-luc showed strong expression of the luc reporter gene lasting up to 3 days postinfection. These studies demonstrate the potential of RCN as a vaccine vector for avian influenza and other poultry pathogens. ?? American Association of Avian Pathologists 2010.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Characterization of recombinant Raccoonpox Vaccine Vectors in Chickens
Series title:
Avian Diseases
DOI:
10.1637/9315-032410-Reg.1
Volume
54
Issue:
4
Year Published:
2010
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
1157
Last page:
1165
Number of Pages:
9