U-Pb ages determined using sensitive high-resolution ion microprobe-reverse geometry on 205 single-grain zircons from 16 ash beds within submarine fan deposits of the Ecca Group provide the first evidence of a marine Permian-Triassic (P-T) boundary in the Karoo Basin of South Africa. These U-Pb ages provide an objective basis for correlating the deep-marine sediments of the southwest Karoo Basin with fluvial-deltaic deposits in the central and eastern parts of the basin where the P-T boundary is recorded in a diverse macrofauna. Furthermore, these new zircon ages and their correlation imply asymmetric subsidence and variable sedimentation rates across the basin. ?? 2009 Geological Society of America.