We document the discovery of Highfin Carpsucker in the Apalachicola River, FL. Three specimens were captured between river kilometer 170-171 on the Apalachicola River in the vicinity of Jim Woodruff Lock and Dam during spring 2007, The specimens were captured within a reach 0.5-1.0 km downstream from the dam and just upstream from a major spawning area for several other catostomids. This is the first record of the species east of the Choctawhatchee River, FL-AL, and is a range extension of 185 km eastward into the Florida Panhandle region of the Northern Gulf of Mexico Coast.