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11-Deoxycortisol is a corticosteroid hormone in the lamprey

Proceedings of the National Academy of Sciences of the United States of America

By:
, , , ,
DOI: 10.1073/pnas.0914026107

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Abstract

Corticosteroid hormones are critical for controlling metabolism, hydromineral balance, and the stress response in vertebrates. Although corticosteroid hormones have been well characterized in most vertebrate groups, the identity of the earliest vertebrate corticosteroid hormone has remained elusive. Here we provide evidence that 11-deoxycortisol is the corticosteroid hormone in the lamprey, a member of the agnathans that evolved more than 500 million years ago. We used RIA, HPLC, and mass spectrometry analysis to determine that 11-deoxycortisol is the active corticosteroid present in lamprey plasma. We also characterized an 11-deoxycortisol receptor extracted from sea lamprey gill cytosol. The receptor was highly specific for 11-deoxycortisol and exhibited corticosteroid binding characteristics, including DNA binding. Furthermore, we observed that 11-deoxycortisol was regulated by the hypothalamus-pituitary axis and responded to acute stress. 11-Deoxycortisol implants reduced sex steroid concentrations and upregulated gill Na+, K+-ATPase, an enzyme critical for ion balance. We show here that 11-deoxycortisol functioned as both a glucocorticoid and a mineralocorticoid in the lamprey. Our findings indicate that a complex and highly specific corticosteroid signaling pathway evolved at least 500 million years ago with the arrival of the earliest vertebrate.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
11-Deoxycortisol is a corticosteroid hormone in the lamprey
Series title:
Proceedings of the National Academy of Sciences of the United States of America
DOI:
10.1073/pnas.0914026107
Volume
107
Issue:
31
Year Published:
2010
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Proceedings of the National Academy of Sciences of the United States of America
First page:
13942
Last page:
13947
Number of Pages:
6