| Abstract: | Methane (CH4) emitted from high-latitude lakes accounts for 2–6% of the global atmospheric CH4 budget. Methanotrophs in lake sediments and water columns mitigate the amount of CH4 that enters the atmosphere, yet their identity and activity in arctic and subarctic lakes are poorly understood. We used stable isotope probing (SIP), quantitative PCR (Q-PCR), pyrosequencing and enrichment cultures to determine the identity and diversity of active aerobic methanotrophs in the water columns and sediments (0–25 cm) from an arctic tundra lake (Lake Qalluuraq) on the north slope of Alaska and a subarctic taiga lake (Lake Killarney) in Alaska‘s interior. The water column CH4 oxidation potential for these shallow (~2m deep) lakes was greatest in hypoxic bottom water from the subarctic lake. The type II methanotroph, Methylocystis, was prevalent in enrichment cultures of planktonic methanotrophs from the water columns. In the sediments, type I methanotrophs (Methylobacter, Methylosoma and Methylomonas) at the sediment-water interface (0–1 cm) were most active in assimilating CH4, whereas the type I methanotroph Methylobacter and/or type II methanotroph Methylocystis contributed substantially to carbon acquisition in the deeper (15–20 cm) sediments. In addition to methanotrophs, an unexpectedly high abundance of methylotrophs also actively utilized CH4-derived carbon. This study provides new insight into the identity and activity of methanotrophs in the sediments and water from high-latitude lakes. |
| Genre: | Article |
| ProdID: | 70038683 |
| Citation Author: | He, Ruo ; Wooller, Matthew J.; Pohlman, John W.; Quensen, John ; Tiedje, James M.; Leigh, Mary Beth |
| Citation Contributing Office: | Woods Hole Coastal and Marine Science Center |
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| Citation End Page: | 1948 |
| Citation Issue: | 10 |
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| Citation Language: | English |
| Citation Larger Work Title: | ISME Journal |
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| Citation Number Of Pages: | 53 |
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| Citation Phsyical Description: | 12 p. |
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| Citation Publisher: | Nature Publishing Group |
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| Citation Search Results Text: | Diversity of active aerobic methanotrophs along depth profiles of arctic and subarctic lake water column and sediments; 2012; Article; Journal; ISME Journal; He, Ruo ; Wooller, Matthew J.; Pohlman, John W.; Quensen, John ; Tiedje, James M.; Leigh, Mary Beth |
| Citation Start Page: | 1937 |
| Citation Volume: | 6 |
| Citation Year: | 2012 |
| Type: | citation/reference |
| Text: | Diversity of active aerobic methanotrophs along depth profiles of arctic and subarctic lake water column and sediments; 2012; Article; Journal; ISME Journal; He, Ruo ; Wooller, Matthew J.; Pohlman, John W.; Quensen, John ; Tiedje, James M.; Leigh, Mary Beth |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| URL (DIGITAL OBJECT IDENTIFIER): | http://dx.doi.org/10.1038/ismej.2012.34 |
| Date Other: | Fri, 27 Jul 2012 00:00 -0500 |
| Publisher: | Nature Publishing Group |
