thumbnail

Human enteric viruses in groundwater indicate offshore transport of human sewage to coral reefs of the Upper Florida Keys

Environmental Microbiology

By:
, ,
DOI: 10.1111/j.1462-2920.2010.02141.x

Links

Abstract

To address the issue of human sewage reaching corals along the main reef of the Florida Keys, samples were collected from surface water, groundwater and coral [surface mucopolysaccharide layers (SML)] along a 10 km transect near Key Largo, FL. Samples were collected semi-annually between July 2003 and September 2005 and processed for faecal indicator bacteria (faecal coliform bacteria, enterococci and Clostridium perfringens) and human-specific enteric viruses (enterovirus RNA and adenovirus DNA) by (RT)-nested polymerase chain reaction. Faecal indicator bacteria concentrations were generally higher nearshore and in the coral SML. Enteric viruses were evenly distributed across the transect stations. Adenoviruses were detected in 37 of 75 samples collected (49.3%) whereas enteroviruses were only found in 8 of 75 samples (10.7%). Both viruses were detected twice as frequently in coral compared with surface water or groundwater. Offshore, viruses were most likely to be found in groundwater, especially during the wet summer season. These data suggest that polluted groundwater may be moving to the outer reef environment in the Florida Keys.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Human enteric viruses in groundwater indicate offshore transport of human sewage to coral reefs of the Upper Florida Keys
Series title:
Environmental Microbiology
DOI:
10.1111/j.1462-2920.2010.02141.x
Volume
12
Issue:
4
Year Published:
2010
Language:
English
Publisher:
Blackwell Publishing
Publisher location:
Malden, MA
Contributing office(s):
Florida Water Science Center-Tallahassee
Description:
11 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
964
Last page:
974
Country:
United States
State:
Florida
Other Geospatial:
Upper Florida Keys