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Characterization of West Nile viruses isolated form captive American flamingoes (Phoenicopterus ruber) in Medellin, Colombia.

American Journal of Tropical Medicine and Hygiene

By:
, , , , , , , , and
DOI: 10.4269/ajtmh.2012.11-0655

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Abstract

Serum samples from a total of 71 healthy captive birds belonging to 18 species were collected in July of 2008 in Medellin (Colombia) and tested for flaviviruses. Eighteen of 29 samples from American Flamingoes (Phoenicopterus ruber) were positive for West Nile virus (WNV) by reverse transcription-polymerase chain reaction. Selected positive samples were serially passaged and WNV was confirmed by immunofluorescence. Two isolates (524/08, 9835/08) were characterized in vitro and in vivo. Sequence analysis revealed WNV with 16 nucleotide substitutions resulting in six amino acid changes when compared with the NY99 strain. Colombian (COL) viruses were more closely related to Louisiana isolates from 2001. When compared with attenuated strains isolated from Texas, COL isolates differed in their plaque size and temperature sensitivity phenotype. The COL viruses were pathogenic in embryonated chicken eggs and Balb/c mice.

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Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Characterization of West Nile viruses isolated form captive American flamingoes (Phoenicopterus ruber) in Medellin, Colombia.
Series title:
American Journal of Tropical Medicine and Hygiene
DOI:
10.4269/ajtmh.2012.11-0655
Volume
87
Issue:
3
Year Published:
2012
Language:
English
Publisher:
American Society of Tropical Medicine and Hygiene
Publisher location:
Deerfield, IL
Contributing office(s):
National Wildlife Health Center
Description:
8 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
American Journal of Tropical Medicine and Hygiene
First page:
565
Last page:
572
Country:
Columbia
City:
Medellin