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Bat white-nose syndrome: a real-time TaqMan polymerase chain reaction test targeting the intergenic spacer region of Geomyces destructanstructans.

Mycologia

By:
, , , , ,
DOI: 10.3852/12-242

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Abstract

The fungus Geomyces destructans is the causative agent of white-nose syndrome (WNS), a disease that has killed millions of North American hibernating bats. We describe a real-time TaqMan PCR test that detects DNA from G. destructans by targeting a portion of the multicopy intergenic spacer region of the rRNA gene complex. The test is highly sensitive, consistently detecting as little as 3.3 fg of genomic DNA from G. destructans. The real-time PCR test specifically amplified genomic DNA from G. destructans but did not amplify target sequence from 54 closely related fungal isolates (including 43 Geomyces spp. isolates) associated with bats. The test was further qualified by analyzing DNA extracted from 91 bat wing skin samples, and PCR results matched histopathology findings. These data indicate the real-time TaqMan PCR method described herein is a sensitive, specific, and rapid test to detect DNA from G. destructans and provides a valuable tool for WNS diagnostics and research.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Bat white-nose syndrome: a real-time TaqMan polymerase chain reaction test targeting the intergenic spacer region of Geomyces destructanstructans.
Series title:
Mycologia
DOI:
10.3852/12-242
Volume
105
Issue:
2
Year Published:
2013
Language:
English
Publisher:
Mycological Society of America
Publisher location:
http://msafungi.org/
Contributing office(s):
National Wildlife Health Center
Description:
7 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Mycologia
First page:
253
Last page:
259