Tools for assessing kinship, population structure, phylogeography, and interspecific hybridization in Asian carps invasive to the Mississippi River, USA: isolation and characterization of novel tetranucleotide microsatellite DNA loci in silver carp Hypophthalmichthys molitrix
Conservation Genetics Resources
By: T.L. King, M.S. Eackles, and D.C. Chapman
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Abstract
We document the isolation and characterization of novel tetranucleotide microsatellite DNA markers for the invasive silver carp Hypophthalmichthys molitrix and provide the results of cross-species amplification for three additional invasive carp species: bighead (H. nobilis), grass (Ctenopharyngodon idella) and black (Mylopharyngodon piceus). In the target species these markers yielded levels of allelic diversity (average 4.4 alleles/locus) and heterozygosity (average 54.7%) sufficient to: (1) provide unique multilocus genotypes; (2) delineate kinship relationships; (3) differentiate populations/species; (4) estimate effective population sizes; and (5) provide unique demographic perspectives for control or eradication. Currently these markers are being utilized to determine the degree of introgressive hybridization between H. molitrix and H. nobilis, to quantify gene flow between different sub-basins established in the central United States, and to assess the demographic status of sub-basin groups. This information will be critically important in the management/control of these invasive species.
Study Area
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Tools for assessing kinship, population structure, phylogeography, and interspecific hybridization in Asian carps invasive to the Mississippi River, USA: isolation and characterization of novel tetranucleotide microsatellite DNA loci in silver carp Hypophthalmichthys molitrix |
| Series title | Conservation Genetics Resources |
| DOI | 10.1007/s12686-010-9285-3 |
| Volume | 3 |
| Issue | 3 |
| Year Published | 2011 |
| Language | English |
| Publisher | Springer |
| Contributing office(s) | Leetown Science Center |
| Description | 5 p. |
| Larger Work Type | Article |
| Larger Work Subtype | Journal Article |
| Larger Work Title | Conservation Genetics Resources |
| First page | 397 |
| Last page | 401 |
| Country | United States |
| Other Geospatial | Mississippi River |
| Google Analytic Metrics | Metrics page |