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Development of polysomic microsatellite markers for characterization of population structuring and phylogeography in the shortnose sturgeon (Acipenser brevirostrum)

Conservation Genetics Resources

By:
,
DOI: 10.1007/s12686-012-9658-x

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Abstract

Shortnose sturgeon Acipenser brevirostrum is an endangered polyploid fish species for which no nuclear DNA markers previously existed. To address this need, 86 polysomic loci were developed and characterized in 20 A. brevirostrum from five river systems and eight members (parents and six progeny) of a captive-bred family. All markers proved to be polymorphic, polysomic, and demonstrated direct inheritance when tested in a captive family. Eleven loci were included in a range-wide survey of 561 fish sampled from 17 geographic collections. Allelic diversity at these markers ranged from 7 to 24 alleles/locus and averaged 16.5 alleles/locus; sufficient diversity to produce unique multilocus genotypes. In the range-wide survey, a Mantel comparison of an ecological (1-Jaccard’s) and genetic (ΦPT; an analog to FST) distance metrics, identified a strong positive correlation (r = 0.98, P < 0.0001); suggesting ΦPT represents a viable metric for assessing genetic relatedness using this class of marker.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Development of polysomic microsatellite markers for characterization of population structuring and phylogeography in the shortnose sturgeon (Acipenser brevirostrum)
Series title:
Conservation Genetics Resources
DOI:
10.1007/s12686-012-9658-x
Volume
4
Issue:
4
Year Published:
2012
Language:
English
Publisher:
Springer
Contributing office(s):
Leetown Science Center
Description:
7 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
853
Last page:
859