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Transcriptional profiling of the parr-smolt transformation in Atlantic salmon

Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics

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DOI: 10.1016/j.cbd.2012.07.003

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Abstract

The parr–smolt transformation in Atlantic salmon (Salmo salar) is a complex developmental process that culminates in the ability to migrate to and live in seawater. We used GRASP 16K cDNA microarrays to identify genes that are differentially expressed in the liver, gill, hypothalamus, pituitary, and olfactory rosettes of smolts compared to parr. Smolts had higher levels of gill Na+/K+-ATPase activity, plasma cortisol and plasma thyroid hormones relative to parr. Across all five tissues, stringent microarray analyses identified 48 features that were differentially expressed in smolts compared to parr. Using a less stringent method we found 477 features that were differentially expressed at least 1.2-fold in smolts, including 172 features in the gill. Smolts had higher mRNA levels of genes involved in transcription, protein biosynthesis and folding, electron transport, oxygen transport, and sensory perception and lower mRNA levels for genes involved in proteolysis. Quantitative RT-PCR was used to confirm differential expression in select genes identified by microarray analyses and to quantify expression of other genes known to be involved in smolting. This study expands our understanding of the molecular processes that underlie smolting in Atlantic salmon and identifies genes for further investigation.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Transcriptional profiling of the parr-smolt transformation in Atlantic salmon
Series title:
Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics
DOI:
10.1016/j.cbd.2012.07.003
Volume
7
Issue:
4
Year Published:
2012
Language:
English
Publisher:
Elsevier
Publisher location:
Amsterdam, Netherlands
Contributing office(s):
Leetown Science Center
Description:
10 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
351
Last page:
360