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Characterization of mannitol in Curvularia protuberata hyphae by FTIR and Raman spectromicroscopy

Analyst

By:
, , , ,
DOI: 10.1039/c0an00534g

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Abstract

FTIR and Raman spectromicroscopy were used to characterize the composition of Curvularia protuberata hyphae, and to compare a strain isolated from plants inhabiting geothermal soils with a non-geothermal isolate. Thermal IR source images of hyphae have been acquired with a 64 × 64 element focal plane array detector; single point IR spectra have been obtained with synchrotron source light. In some C. protuberata hyphae, we have discovered the spectral signature of crystalline mannitol, a fungal polyol with complex protective roles. With FTIR-FPA imaging, we have determined that the protein content in cells remains fairly constant throughout the length of a hypha, whereas the mannitol is found at discrete, irregular locations. This is the first direct observation of mannitol in intact fungal hyphae. Since the concentration of mannitol in cells varies with respect to position and is not present in all hyphae, this discovery may be related to habitat adaptation, fungal structure and growth stages.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Characterization of mannitol in Curvularia protuberata hyphae by FTIR and Raman spectromicroscopy
Series title:
Analyst
DOI:
10.1039/c0an00534g
Volume
135
Issue:
12
Year Published:
2010
Language:
English
Publisher:
RSC Publishing
Contributing office(s):
Western Fisheries Research Center
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
3249
Last page:
3254
Number of Pages:
6
Country:
United States