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Development and characterization of 21 polymorphic microsatellite markers for the barren-ground shrew, Sorex ugyunak (Mammalia: Sorcidae), through next-generation sequencing, and cross-species amplification in the masked shrew, S. cinereus

Conservation Genetics Resources

By:
, , , , ,
DOI: 10.1007/s12686-012-9792-5

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Abstract

We used next generation shotgun sequencing to develop 21 novel microsatellite markers for the barren-ground shrew (Sorex ugyunak), which were polymorphic among individuals from northern Alaska. The loci displayed moderate allelic diversity (averaging 6.81 alleles per locus) and heterozygosity (averaging 70 %). Two loci deviated from Hardy–Weinberg equilibrium (HWE) due to heterozygote deficiency. While the population did not deviate from HWE overall, it showed significant linkage disequilibrium suggesting this population is not in mutation-drift equilibrium. Nineteen of 21 loci were polymorphic in masked shrews (S. cinereus) from interior Alaska and exhibited linkage equilibrium and HWE overall. All loci yielded sufficient variability for use in population studies.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Development and characterization of 21 polymorphic microsatellite markers for the barren-ground shrew, Sorex ugyunak (Mammalia: Sorcidae), through next-generation sequencing, and cross-species amplification in the masked shrew, S. cinereus
Series title:
Conservation Genetics Resources
DOI:
10.1007/s12686-012-9792-5
Volume
5
Issue:
2
Year Published:
2013
Language:
English
Publisher:
Springer
Contributing office(s):
Alaska Science Center Biology
Description:
4 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Conservation Genetics Resources
First page:
315
Last page:
318