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Characterization of the OmyY1 region on the rainbow trout Y chromosome

International Journal of Genomics

By:
, , , , , , ,
DOI: 10.1155/2013/261730

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Abstract

We characterized the male-specific region on the Y chromosome of rainbow trout, which contains both sdY (the sex-determining gene) and the male-specific genetic marker, OmyY1. Several clones containing the OmyY1 marker were screened from a BAC library from a YY clonal line and found to be part of an 800 kb BAC contig. Using fluorescence in situ hybridization (FISH), these clones were localized to the end of the short arm of the Y chromosome in rainbow trout, with an additional signal on the end of the X chromosome in many cells. We sequenced a minimum tiling path of these clones using Illumina and 454 pyrosequencing. The region is rich in transposons and rDNA, but also appears to contain several single-copy protein-coding genes. Most of these genes are also found on the X chromosome; and in several cases sex-specific SNPs in these genes were identified between the male (YY) and female (XX) homozygous clonal lines. Additional genes were identified by hybridization of the BACs to the cGRASP salmonid 4x44K oligo microarray. By BLASTn evaluations using hypothetical transcripts of OmyY1-linked candidate genes as query against several EST databases, we conclude at least 12 of these candidate genes are likely functional, and expressed.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Characterization of the OmyY1 region on the rainbow trout Y chromosome
Series title:
International Journal of Genomics
DOI:
10.1155/2013/261730
Volume
2013
Year Published:
2013
Language:
English
Publisher:
Hindawi Publishing Corporation
Publisher location:
Cairo, Egypt
Contributing office(s):
Western Fisheries Research Center
Description:
261730; 10 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
International Journal of Genomics