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The relative contribution of methanotrophs to microbial communities and carbon cycling in soil overlying a coal-bed methane seep

FEMS Microbiology Ecology

By:
, , , , , ,
DOI: 10.1111/1574-6941.12079

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Abstract

Seepage of coal-bed methane (CBM) through soils is a potential source of atmospheric CH4 and also a likely source of ancient (i.e. 14C-dead) carbon to soil microbial communities. Natural abundance 13C and 14C compositions of bacterial membrane phospholipid fatty acids (PLFAs) and soil gas CO2 and CH4 were used to assess the incorporation of CBM-derived carbon into methanotrophs and other members of the soil microbial community. Concentrations of type I and type II methanotroph PLFA biomarkers (16:1ω8c and 18:1ω8c, respectively) were elevated in CBM-impacted soils compared with a control site. Comparison of PLFA and 16s rDNA data suggested type I and II methanotroph populations were well estimated and overestimated by their PLFA biomarkers, respectively. The δ13C values of PLFAs common in type I and II methanotrophs were as negative as −67‰ and consistent with the assimilation of CBM. PLFAs more indicative of nonmethanotrophic bacteria had δ13C values that were intermediate indicating assimilation of both plant- and CBM-derived carbon. Δ14C values of select PLFAs (−351 to −936‰) indicated similar patterns of CBM assimilation by methanotrophs and nonmethanotrophs and were used to estimate that 35–91% of carbon assimilated by nonmethanotrophs was derived from CBM depending on time of sampling and soil depth.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
The relative contribution of methanotrophs to microbial communities and carbon cycling in soil overlying a coal-bed methane seep
Series title:
FEMS Microbiology Ecology
DOI:
10.1111/1574-6941.12079
Volume
84
Issue:
3
Year Published:
2013
Language:
English
Publisher:
Wiley
Contributing office(s):
Crustal Geophysics and Geochemistry Science Center
Description:
21 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
474
Last page:
494