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Detection of Mycoplasma agassizii in the Texas Tortoise (Gopherus berlandieri)

Journal of Wildlife Diseases

By:
, , ,
DOI: 10.7589/2012-07-181

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Abstract

Mycoplasma agassizii causes upper respiratory tract disease (URTD) in Texas tortoises (Gopherus berlandieri). To determine exposure to and shedding of M. agassizii, we collected blood samples and nasal swabs from 40 free-ranging Texas tortoises on public and private lands in Texas, USA, from May to October 2009. We used an enzyme-linked immunosorbent assay (ELISA) to detect M. agassizii–specific antibodies. Eleven (28%) tortoises were antibody positive, three (8%) were suspect, and the remaining 26 (65%) were negative. Nasal lavage samples were collected from 35 of the 40 tortoises for M. agassizii culture and PCR to detect shedding of M. agassizii. Current infection with M. agassizii was confirmed in one tortoise that had mild clinical signs of URTD and was positive by ELISA (antibody titer >512), PCR, and culture. The clinical isolate was confirmed as M. agassizii by restriction fragment length polymorphism and immunobinding.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Detection of Mycoplasma agassizii in the Texas Tortoise (Gopherus berlandieri)
Series title:
Journal of Wildlife Diseases
DOI:
10.7589/2012-07-181
Volume
49
Issue:
3
Year Published:
2013
Language:
English
Publisher:
Wildlife Disease Association
Contributing office(s):
National Wildlife Health Center
Description:
5 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
704
Last page:
708
Number of Pages:
5