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Blood from a turnip: tissue origin of low-coverage shotgun sequencing libraries affects recovery of mitogenome sequences

Mitochondrial DNA

By:
, , and
DOI: 10.3109/19401736.2013.840588

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Abstract

Next generation sequencing methods allow rapid, economical accumulation of data that have many applications, even at relatively low levels of genome coverage. However, the utility of shotgun sequencing data sets for specific goals may vary depending on the biological nature of the samples sequenced. We show that the ability to assemble mitogenomes from three avian samples of two different tissue types varies widely. In particular, data with coverage typical of microsatellite development efforts (1×) from DNA extracted from avian blood failed to cover even 50% of the mitogenome, relative to at least 500-fold coverage from muscle-derived data. Researchers should consider possible applications of their data and select the tissue source for their work accordingly. Practitioners analyzing low-coverage shotgun sequencing data (including for microsatellite locus development) should consider the potential benefits of mitogenome assembly, including internal barcode verification of species identity, mitochondrial primer development, and phylogenetics.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Blood from a turnip: tissue origin of low-coverage shotgun sequencing libraries affects recovery of mitogenome sequences
Series title:
Mitochondrial DNA
DOI:
10.3109/19401736.2013.840588
Edition:
Online First
Year Published:
2013
Language:
English
Publisher:
Informa Healthcare
Contributing office(s):
Fort Collins Science Center
Description:
5 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Mitochondrial DNA
First page:
1
Last page:
5
Number of Pages:
5
Online Only (Y/N):
Y