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TanA: a fluorogenic probe for thiaminase activity

Analytical Methods

By:
, , , and
DOI: 10.1039/C2AY26047F

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Abstract

A fluorogenic thiamine analogue is presented as a fluorescent probe for thiaminase activity. The emission of the fluorophore is quenched by photoinduced electron transfer (PET) to the N-substituted pyridinium portion of the probe. Action of the enzyme releases the free pyridine group causing a substantial increase in fluorescence.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
TanA: a fluorogenic probe for thiaminase activity
Series title:
Analytical Methods
DOI:
10.1039/C2AY26047F
Volume
5
Issue:
2
Year Published:
2013
Language:
English
Publisher:
RSC Publishing
Contributing office(s):
Columbia Environmental Research Center
Description:
3 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Analytical Methods
First page:
446
Last page:
448