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Pheromonal bile acid 3-ketopetromyzonol sulfate primes the neuroendocrine system in sea lamprey

BMC Neuroscience

By:
, , , , , ,
DOI: 10.1186/1471-2202-14-11

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Abstract

Background Vertebrate pheromones are known to prime the endocrine system, especially the hypothalamic-pituitary-gonadal (HPG) axis. However, no known pheromone molecule has been shown to modulate directly the synthesis or release of gonadotropin releasing hormone (GnRH), the main regulator of the HPG axis. We selected sea lamprey (Petromyzon marinus) as a model system to determine whether a single pheromone component alters the output of GnRH. Sea lamprey male sex pheromones contain a main component, 7α, 12α, 24-trihydroxy-5α-cholan-3-one 24-sulfate (3 keto-petromyzonol sulfate or 3kPZS), which has been shown to modulate behaviors of mature females. Through a series of experiments, we tested the hypothesis that 3kPZS modulates both synthesis and release of GnRH, and subsequently, HPG output in immature sea lamprey. Results The results showed that natural male pheromone mixtures induced differential steroid responses but facilitated sexual maturation in both sexes of immature animals (χ2 = 5.042, dF = 1, p < 0.05). Exposure to 3kPZS increased plasma 15α-hydroxyprogesterone (15α-P) concentrations (one-way ANOVA, p < 0.05) and brain gene expressions (genes examined: three lamprey (l) GnRH-I transcripts, lGnRH-III, Jun and Jun N-terminal kinase (JNK); one-way ANOVA, p < 0.05), but did not alter the number of GnRH neurons in the hypothalamus in immature animals. In addition, 3kPZS treatments increased lGnRH peptide concentrations in the forebrain and modulated their levels in plasma. Overall, 3kPZS modulation of HPG axis is more pronounced in immature males than in females. Conclusions We conclude that a single male pheromone component primes the HPG axis in immature sea lamprey in a sexually dimorphic manner.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Pheromonal bile acid 3-ketopetromyzonol sulfate primes the neuroendocrine system in sea lamprey
Series title:
BMC Neuroscience
DOI:
10.1186/1471-2202-14-11
Volume
14
Issue:
11
Year Published:
2013
Language:
English
Publisher:
BioMed Central
Contributing office(s):
Great Lakes Science Center
Description:
13 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
BMC Neuroscience
Number of Pages:
13
Online Only (Y/N):
Y