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In vitro immune functions in thiamine-replete and -depleted lake trout (Salvelinus namaycush)

Fish & Shellfish Immunology

By:
, , ,
DOI: 10.1016/j.fsi.2014.03.024

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Abstract

In this study we examined the impacts of in vivo thiamine deficiency on lake trout leukocyte function measured in vitro. When compared outside the context of individual-specific thiamine concentrations no significant differences were observed in leukocyte bactericidal activity or in concanavalin A (Con A), and phytohemagglutinin-P (PHA-P) stimulated leukocyte proliferation. Placing immune functions into context with the ratio of in vivo liver thiamine monophosphate (TMP – biologically inactive form) to thiamine pyrophosphate (TPP – biologically active form) proved to be the best indicator of thiamine depletion impacts as determined using regression modeling. These observed relationships indicated differential effects on the immune measures with bactericidal activity exhibiting an inverse relationship with TMP to TPP ratios, Con A stimulated mitogenesis exhibiting a positive relationship with TMP to TPP ratios and PHA-P stimulated mitogenesis exhibiting no significant relationships. In addition, these relationships showed considerable complexity which included the consistent observation of a thiamine-replete subgroup with characteristics similar to those seen in the leukocytes from thiamine-depleted fish. When considered together, our observations indicate that lake trout leukocytes experience cell-type specific impacts as well as an altered physiologic environment when confronted with a thiamine-limited state.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
In vitro immune functions in thiamine-replete and -depleted lake trout (Salvelinus namaycush)
Series title:
Fish & Shellfish Immunology
DOI:
10.1016/j.fsi.2014.03.024
Volume
38
Issue:
1
Year Published:
2014
Language:
English
Publisher:
Elsevier
Contributing office(s):
Leetown Science Center
Description:
10 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
211
Last page:
220
Number of Pages:
10