Concentration of infectious hematopoietic necrosis virus from water samples by tangential flow filtration and polyethylene glycol precipitation
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Abstract
Infectious hematopoietic necrosis virus (IHNV) was concentrated from water samples by polyethylene glycol (PEG) precipitation, tangential flow filtration (TFF), and by a combination of TFF followed by PEG precipitation of the retentate. Used alone, PEG increased virus titers more than 200-fold, and the efficiency of recovery was as great as 100%. Used alone, TFF concentrated IHNV more than 20-fold, and average recovery was 70%. When the two techniques were combined, 10-L water samples were reduced to about 300 mL by TFF and the virus was precipitated with PEG into a 1 to 2 g pellet; total recovery was as great as 100%. The combined techniques were used to isolate IHNV from water samples taken from a river containing adult sockeye salmon (Oncorhynchus nerka) and from a hatchery pond containing adult spring chinook salmon (O. tshawytscha). The combination of these methods was effective in concentrating and detecting IHNV from water containing only three infectious particles per 10-L sample.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Concentration of infectious hematopoietic necrosis virus from water samples by tangential flow filtration and polyethylene glycol precipitation |
| Series title | Canadian Journal of Fisheries and Aquatic Sciences |
| DOI | 10.1139/f89-125 |
| Volume | 46 |
| Issue | 6 |
| Year Published | 1989 |
| Language | English |
| Publisher | Canadian Science Publishing |
| Contributing office(s) | Western Fisheries Research Center |
| Description | 5 p. |
| First page | 964 |
| Last page | 968 |
| Google Analytic Metrics | Metrics page |