Survival of the North American strain of viral hemorrhagic septicemia virus (VHSV) in filtered seawater and seawater containing ovarian fluid, crude oil and serum-enriched culture medium
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Abstract
The North American strain of viral hemorrhagic septicemia virus (NA-VHSV) could be recovered for up to 40 h in natural filtered seawater (27 ppt) with a 50% loss of infectivity after approximately 10 h at 15°C. Addition of 10 ppb North Slope crude oil to the seawater had no effect on virus survival. However, when various concentrations of teleost ovarian fluid were added to seawater, virus could be recovered after 72 h at 0.01% ovarian fluid and after 96 h at 1.0%. When cell culture medium supplemented with 10% fetal bovine serum was added to the seawater, 100% of the virus could be recovered for the first 15 d and 60% of the virus remained after 36 d. These findings quantify NA-VHSV infectivity in natural seawater and demonstrate that ovarian fluid, which occurs naturally during spawning events, significantly prolongs the survival and infectivity of the virus. The extended stabilization of virus in culture medium supplemented with serum allows for low titer field samples to be collected and transported in an unfrozen state without significant loss of virus titer.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Survival of the North American strain of viral hemorrhagic septicemia virus (VHSV) in filtered seawater and seawater containing ovarian fluid, crude oil and serum-enriched culture medium |
| Series title | Diseases of Aquatic Organisms |
| DOI | 10.3354/dao044075 |
| Volume | 44 |
| Issue | 1 |
| Year Published | 2001 |
| Language | English |
| Publisher | Inter-Research |
| Contributing office(s) | Western Fisheries Research Center |
| Description | 4 p. |
| First page | 75 |
| Last page | 78 |
| Online Only (Y/N) | N |
| Additional Online Files (Y/N) | N |
| Google Analytic Metrics | Metrics page |