Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques

Mariano Carossino; Hon S. Ip; Jürgen a. Richt; Kendra Schultz; Kimberly Harper; Alan T. Loynachan; Fabio Del Piero; Udeni B.R. Balasuriya

doi:10.1007/s00705-020-04737-w

Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques

Archives of Virology

By: Mariano Carossino, Hon S. Ip, Jürgen a. Richt, Kendra Schultz, Kimberly Harper, Alan T. Loynachan, Fabio Del Piero, and Udeni B.R. Balasuriya

https://doi.org/10.1007/s00705-020-04737-w

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Abstract

In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope^®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.

Additional publication details
Publication type	Article
Publication Subtype	Journal Article
Title	Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques
Series title	Archives of Virology
DOI	10.1007/s00705-020-04737-w
Volume	165
Year Published	2020
Contributing office(s)	National Wildlife Health Center
Description	5 p.
First page	2373
Last page	2377
Google Analytic Metrics	Metrics page