Production and characterization of a mouse monoclonal antibody against smallmouth bass (Micropterus dolomieu) IgM
Fish & Shellfish Immunology
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Abstract
A murine monoclonal antibody (mAb, IgG2a) was produced for the detection of smallmouth bass (Micropterus dolomieu) immunoglobulin (IgM). The antibody is specific for IgM heavy chain and was shown to also recognize the Ig heavy chain of largemouth bass (Micropterus salmoides) and bluegill (Lepomis macrochirus) using Western Blot analysis of plasma from 9 teleost taxa. When applied to the analysis of smallmouth bass total plasma IgM using ELISA, the mAb was found to be effective when used in an inhibition kinetic assay.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Production and characterization of a mouse monoclonal antibody against smallmouth bass (Micropterus dolomieu) IgM |
| Series title | Fish & Shellfish Immunology |
| DOI | 10.1016/j.fsi.2021.03.006 |
| Volume | 113 |
| Year Published | 2021 |
| Language | English |
| Publisher | Elsevier |
| Contributing office(s) | Leetown Science Center, Eastern Ecological Science Center |
| Description | 4 p. |
| First page | 20 |
| Last page | 23 |
| Google Analytic Metrics | Metrics page |