This report presents water-quality and biologic data collected in the Blue River Basin, metropolitan Kansas City, Missouri and Kansas, from October 2000 to October 2004. Data were collected in cooperation with the city of Kansas City, Missouri, Water Services Department as part of an ongoing study designed to characterize long-term water-quality trends in the basin and to provide data to support a strategy for combined sewer overflow control. These data include values of physical properties, fecal indicator bacteria densities, suspended sediment, and concentrations of major ions, nutrients, trace elements, organic wastewater compounds, and pharmaceutical compounds in base-flow and stormflow stream samples and bottom sediments. Six surface-water sites in the basin were sampled 13 times during base-flow conditions and during a minimum of 7 storms. Benthic macroinvertebrate communities are described at 10 sites in the basin and 1 site outside the basin. Water-column and bottom-sediment data from impounded reaches of Brush Creek are provided. Continuous specific conductance, pH, water-quality temperature, turbidity, and dissolved oxygen data are provided for two streams-the Blue River and Brush Creek. Sampling, analytical, and quality assurance methods used in data collection during the study also are described in the report.