The U.S. Geological Survey collected hydrologic data between June 2004 and December 2005 to investigate the temporal and spatial nature of flow exchanges through culverts beneath State Road 9336 within Everglades National Park. Continuous data collected during the study measured flow velocity, water level, salinity, conductivity, and water-temperature in or near seven culverts between Pa-hay-okee Overlook access road and Nine Mile Pond. The two culverts east of Pa-hay-okee Overlook access road flowed into Taylor Slough Basin from 87 to 96 percent of the study period, whereas flows through five culverts between Pa-hay-okee Overlook access road and Nine Mile Pond flowed into Shark River Slough Basin from 70 to 99 percent of the study period. Synoptic flow discharges measured at all culverts during three intensive field efforts revealed a net discharge into Taylor Slough Basin from Shark River Slough Basin through culverts between Royal Palm Road and Pa-hay-okee Overlook access road, and into Shark River Slough Basin from Taylor Slough Basin through culverts between Pa-hay-okee Overlook access road and Nine Mile Pond. Data collected during the study and presented in this report provided additional knowledge of the magnitude, direction, and nature of flow exchanges through the road culverts.