| Abstract: | Age distribution information can be an important part of understanding the biology of any population. Age estimates collected from the annual growth rings found in tooth and bone cross sections, often referred to as Lines of Arrested Growth (LAGs), have been used in the study of various animals. In this manual, we describe in detail all necessary steps required to obtain estimates of age from anuran bone cross sections via skeletochronological assessment. We include comprehensive descriptions of how to fix and decalcify toe specimens (phalanges), process a phalange prior to embedding, embed the phalange in paraffin, section the phalange using a microtome, stain and mount the cross sections of the phalange and read the LAGs to obtain age estimates. |
| Genre: | USGS Numbered Series |
| ProdID: | 97051 |
| Citation Author: | McCreary, Brome; Pearl, Christopher A.; Adams, Michael J. |
| Citation Contributing Office: | Geological Survey (U.S.) |
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| Citation Language: | ENGLISH |
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| Citation Phsyical Description: | iv, 38 p. |
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| Citation Publisher: | Geological Survey (U.S.) |
| Citation Series: | Open-File Report |
| Citation Series Code: | OFR |
| Citation Series Number: | 2008-1209 |
| Citation Search Results Text: | A Protocol for Aging Anurans Using Skeletochronology; 2008; OFR; 2008-1209; McCreary, Brome; Pearl, Christopher A.; Adams, Michael J. |
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| Citation Year: | 2008 |
| Type: | citation/reference |
| Text: | A Protocol for Aging Anurans Using Skeletochronology; 2008; OFR; 2008-1209; McCreary, Brome; Pearl, Christopher A.; Adams, Michael J. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/usgs_thumb.jpg |
| URL (INDEX PAGE): | http://pubs.usgs.gov/of/2008/1209/ |
| Date Other: | Sat, 25 Oct 2008 00:00 -0500 |
| Publisher: | Geological Survey (U.S.) |
