Construction of a section of Interstate Highway 77 through Glade Creek basin in Wythe County, southwestern Virginia, raised concern about its effect on two springs and a well that supply water to a fish hatchery in the basin. Discharge of Glade Creek and the two springs, turbidity of the springs, water levels in the well, water temperature, and chemical quality of the springs and well were monitored during construction and for 6 months after completion of the highway. Analyses of the data showed no trends, anomalies, or changes in quality of water that could be related either to construction activities or to the presence and use of the completed highway.