Knowledge of the low-flow characteristics of Florida streams and rivers is essential in planning for the availability of adequate quantities of water for commercial- and public-water supply, agricultural irrigation, artificial recharge, and the dilution of waste discharge. This report provides low-flow characteristics for 216 continuous-record gaging stations using frequency analysis techniques. Included are low-flow frequency characteristics for 143 unregulated, gaging stations; and sample percentiles for 32 stations that were subject to regulation or diversion, and sample percentiles for 41 stations, regulated and unregulated, that exhibited significant trends in the annual low-flow time series. Estimates of low-flow frequency characteristics are provided for 242 partial-record stations and miscellaneous sites based on correlations with daily mean discharges at continuous-record stations. Low-flow measurement data are available at approximately 1,300 continuous-record gaging stations, partial- record stations and miscellaneous sites. Historic low-flow measurement data are accessible through the U.S. Geological Survey Automatic Data Processing System.