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Preparative free-flow electrophoresis as a method of fractionation of natural organic materials

Water Supply Paper 1817-D

By:
,

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Abstract

Preparative free-flow electrophoresis was found to be an efficient method of conducting large-scale fractionations of the natural organic polyelectrolytes occurring in many surface waters and soils. The method of free-flow electrophoresis obviates, the problem of adsorption upon a supporting medium and permits the use of high potential gradients and currents because of an efficient cooling system. Separations were monitored by determining organic carbon concentration with a dissolved carbon analyzer, and color was measured by absorbance at 400 nanometers. Organic materials from waters and soils were purified by filtration, hydrogen exchange, and dialysis and were concentrated by freeze drying or freeze concentration. In electrophoretic fractionations of natural organic materials typically found in surface waters and soils, color was found to increase with the charge of the fraction.

Additional Publication Details

Publication type:
Report
Publication Subtype:
USGS Numbered Series
Title:
Preparative free-flow electrophoresis as a method of fractionation of natural organic materials
Series title:
Water Supply Paper
Series number:
1817
Chapter:
D
Edition:
-
Year Published:
1973
Language:
ENGLISH
Publisher:
U.S. Govt. Print. Off.,
Description:
iii, 14 p. :illus. ;24 cm.