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In vitro toxicity and interactions of environmental contaminants (Arochlor 1254 and mercury) and immunomodulatory agents (lipopolysaccharide and cortisol) on thymocytes from lake trout (Salvelinus namaycush)

Fish and Shellfish Immunology

By:
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DOI: 10.1006/fsim.2001.0381

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Abstract

The immunotoxicity of chemical combinations commonly encountered by the lake trout (Salvelinus namaycush) immune system was the focus of this study. It was hypothesised that combinations of an environmental contaminant (mercuric chloride or Aroclor 1254) and an immunomodulatory agent (bacterial endotoxin or cortisol) might interact to produce a greater toxicity than that of the environmental contaminant alone at concentrations typically encountered in piscine blood and other tissues. Thus lake trout thymocytes were isolated and treated with mercuric chloride or Aroclor 1254 in the presence and absence of cortisol or lipopolysaccharide. Incubations were performed for 6 or 20 h at 4° C or 10° C. Lipopolysaccharide did not affect the toxicity of either contaminant. In contrast, cortisol enhanced the toxicity of both environmental contaminants. Hence, stressors that lead to increased cortisol production, but not lipopolysaccharide directly, may increase the toxicity of mercury and Aroclor 1254 to lake trout thymocytes.

Additional publication details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
In vitro toxicity and interactions of environmental contaminants (Arochlor 1254 and mercury) and immunomodulatory agents (lipopolysaccharide and cortisol) on thymocytes from lake trout (Salvelinus namaycush)
Series title:
Fish and Shellfish Immunology
DOI:
10.1006/fsim.2001.0381
Volume:
13
Issue:
1
Year Published:
2002
Language:
English
Publisher:
Elsevier
Contributing office(s):
Great Lakes Science Center
Description:
16 p.
First page:
11
Last page:
26
Online Only (Y/N):
N
Additional Online Files (Y/N):
N