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Rapid method for measuring rotenone in water at piscicidal concentrations

Transactions of the American Fisheries Society
By: , and 

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Abstract

A high-performance liquid chromatography (HPLC) procedure that is rapid, specific, and sensitive (limit of detection <0.005 mg/liter) was developed for monitoring application and degradation rates of rotenone. For analysis, a water sample is buffered to pH 5 and injected through a Sep Pak(R) C18 disposable cartridge. The cartridge adsorbs and retains the rotenone which then can be eluted quantitatively from the cartridge with a small volume of methanol. This step effectively concentrates the sample and provides sample cleanup. The methanol extract is analyzed directly by HPLC on an MCH 10 reverse-phase column; methanol: water (75:25, volume : volume) is the mobile phase and flow rate is 1.5 ml/minute. The rotenone is detected by ultraviolet spectrophotometry at a wavelength of 295 nm.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Rapid method for measuring rotenone in water at piscicidal concentrations
Series title Transactions of the American Fisheries Society
Volume 112
Issue 5
Year Published 1983
Language English
Contributing office(s) Upper Midwest Environmental Sciences Center
Description pp. 725-727
Larger Work Type Article
Larger Work Subtype Journal Article
Larger Work Title Transactions of the American Fisheries Society
First page 725
Last page 727