Rapid method for measuring rotenone in water at piscicidal concentrations
Transactions of the American Fisheries Society
By: V. K. Dawson, P.D. Harman, D.P. Schultz, and J. L. Allen
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Abstract
A high-performance liquid chromatography (HPLC) procedure that is rapid, specific, and sensitive (limit of detection <0.005 mg/liter) was developed for monitoring application and degradation rates of rotenone. For analysis, a water sample is buffered to pH 5 and injected through a Sep Pak(R) C18 disposable cartridge. The cartridge adsorbs and retains the rotenone which then can be eluted quantitatively from the cartridge with a small volume of methanol. This step effectively concentrates the sample and provides sample cleanup. The methanol extract is analyzed directly by HPLC on an MCH 10 reverse-phase column; methanol: water (75:25, volume : volume) is the mobile phase and flow rate is 1.5 ml/minute. The rotenone is detected by ultraviolet spectrophotometry at a wavelength of 295 nm.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Rapid method for measuring rotenone in water at piscicidal concentrations |
| Series title | Transactions of the American Fisheries Society |
| Volume | 112 |
| Issue | 5 |
| Year Published | 1983 |
| Language | English |
| Contributing office(s) | Upper Midwest Environmental Sciences Center |
| Description | pp. 725-727 |
| Larger Work Type | Article |
| Larger Work Subtype | Journal Article |
| Larger Work Title | Transactions of the American Fisheries Society |
| First page | 725 |
| Last page | 727 |
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