Liquid-chromatographic determination of sarafloxacin residues in channel catfish muscle-tissue
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Abstract
A liquid chromatographic method is described for the determination of sarafloxacin hydrochloride residues in channel catfish (Ictalurus punctatus) fillets. Sarafloxacin was extracted from fillet tissue with acetonitrile–water (1+1). The extract was centrifuged and the supernatant was partitioned with hexane. The aqueous fraction was filtered through a 0.45 μm filter and evaporated to dryness. The sample was redissolved with 20% acetonitrile–methanol (3 + 2) and 80% trifluoroacetic acid (0.1%), centrifuged, and filtered to remove proteins. Samples were analyzed by chromatography with gradient elution on a C18 column and with fluorescence detection (excitation at 280 nm and emission above 389 nm). Mean recoveries ranged from 85.4 to 104%, and relative standard deviations ranged from 1.06 to 5.58% in samples spiked at concentrations of 10.0–863.8 ng/g. The method detection limit for sarafloxacin was 1.4 ng/g.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Liquid-chromatographic determination of sarafloxacin residues in channel catfish muscle-tissue |
| Series title | Journal of AOAC International |
| DOI | 10.1093/jaoac/77.4.871 |
| Volume | 77 |
| Issue | 4 |
| Year Published | 1994 |
| Language | English |
| Publisher | Oxford Academic |
| Contributing office(s) | Upper Midwest Environmental Sciences Center |
| Description | 5 p. |
| First page | 871 |
| Last page | 875 |
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