Liquid-chromatographic determination of sarafloxacin residues in channel catfish muscle-tissue
Links
- More information: Publisher Index Page (via DOI)
- Open Access Version: Publisher Index Page
- Download citation as: RIS | Dublin Core
Abstract
A liquid chromatographic method is described for the determination of sarafloxacin hydrochloride residues in channel catfish (Ictalurus punctatus) fillets. Sarafloxacin was extracted from fillet tissue with acetonitrile–water (1+1). The extract was centrifuged and the supernatant was partitioned with hexane. The aqueous fraction was filtered through a 0.45 μm filter and evaporated to dryness. The sample was redissolved with 20% acetonitrile–methanol (3 + 2) and 80% trifluoroacetic acid (0.1%), centrifuged, and filtered to remove proteins. Samples were analyzed by chromatography with gradient elution on a C18 column and with fluorescence detection (excitation at 280 nm and emission above 389 nm). Mean recoveries ranged from 85.4 to 104%, and relative standard deviations ranged from 1.06 to 5.58% in samples spiked at concentrations of 10.0–863.8 ng/g. The method detection limit for sarafloxacin was 1.4 ng/g.
Publication type | Article |
---|---|
Publication Subtype | Journal Article |
Title | Liquid-chromatographic determination of sarafloxacin residues in channel catfish muscle-tissue |
Series title | Journal of AOAC International |
DOI | 10.1093/jaoac/77.4.871 |
Volume | 77 |
Issue | 4 |
Year Published | 1994 |
Language | English |
Publisher | Oxford Academic |
Contributing office(s) | Upper Midwest Environmental Sciences Center |
Description | 5 p. |
First page | 871 |
Last page | 875 |
Google Analytic Metrics | Metrics page |