A cryopreservation method for Pasteurella multocida from wetland samples
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Abstract
A cryopreservation method and improved isolation techniques for detection of Pasteurella multocida from wetland samples were developed. Wetland water samples were collected in the field, diluted in dimethyl sulfoxide (DMSO, final concentration 10%), and frozen at -180 C in a liquid nitrogen vapor shipper. Frozen samples were transported to the laboratory where they were subsequently thawed and processed in Pasteurella multocida selective broth (PMSB) to isolate P. multocida. This method allowed for consistent isolation of 2 to 18 organisms/ml from water seeded with known concentrations of P. multocida. The method compared favorably with the standard mouse inoculation method and allowed for preservation of the samples until they could be processed in the laboratory.
Study Area
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | A cryopreservation method for Pasteurella multocida from wetland samples |
| Series title | Journal of Wildlife Diseases |
| DOI | 10.7589/0090-3558-34.1.182 |
| Volume | 34 |
| Issue | 1 |
| Year Published | 1998 |
| Language | English |
| Publisher | Wildlife Disease Association |
| Contributing office(s) | National Wildlife Health Center |
| Description | 4 p. |
| First page | 182 |
| Last page | 185 |
| Country | United States |
| State | California, Nebraska, Nevada |
| Online Only (Y/N) | N |
| Additional Online Files (Y/N) | N |
| Google Analytic Metrics | Metrics page |