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Dinucleotide repeat polymorphisms in waterfowl (family Anatidae): Characterization of a sex-linked (Z-specific) and 14 autosomal loci

Animal Genetics

By:
, , , and
DOI:10.1046/j.1365-2052.1998.00247.x

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Abstract

Canada goose (Branta Canadensis) and harlequin duck (Histrionicus histrionicus) DNAs were digested with Sau3AI, and size selected (300-700 bp) fragments were ligated into BamHI-digested pBluscriptII KS+. The enrichment protocol of Ostrander et al.1 was followed. The resulting libraries were screened using a [ƴ-32P]ATP end-labelled (CA)20 oligonucleotides as a hybridization probe. Positive clones were sequenced using cycle-sequencing protocols (Epicentre Technologies, Madison, WI) and primers flanking the inserts. PCR primers were designed to amplify the repeat and yield amplification products of ≈100-200 bp. DNA  samples were screened for variation at these loci using [ƴ-32P]ATP end-labelled primers. The products were resolved using 6% denaturing polyacrylamide gels and autoradiography.

Additional publication details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Dinucleotide repeat polymorphisms in waterfowl (family Anatidae): Characterization of a sex-linked (Z-specific) and 14 autosomal loci
Series title:
Animal Genetics
DOI:
10.1046/j.1365-2052.1998.00247.x
Volume:
29
Issue:
4
Year Published:
1998
Language:
English
Publisher:
Wiley
Contributing office(s):
Alaska Biological Science Center, Alaska Science Center
Description:
2 p.
First page:
323
Last page:
325