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Thymocyte plasma membrane of the rainbow trout, Salmo gairdneri: Associated immunoglobulin and heteroantigens

Comparative Biochemistry and Physiology, Part B: Comparative Biochemistry

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, , and
https://doi.org/10.1016/0305-0491(83)90285-7

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Abstract

1. Thymic lymphocytes of the rainbow trout, S. gairdneri were disrupted and a plasma membrane containing fraction isolated by differential and buoyant density centrifugation.

2. Radioiodine introduced into the membrane by the lactoperoxidase catalyzed reaction and immunoglobulin (identified by radioimmunoassay with monoclonal antibody) both copurified in the plasma membrane fraction.

3. Rabbit antibody raised to the plasma membrane fraction showed a strong reaction with trout lymphocytes in immunofluorescence, was mitogenic for trout lymphocytes, and recognized lymphocyte membrane heteroantigens of molecular weight > 70,000 in the thymus and 45,000–95,000 in the head kidney.

Additional publication details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Thymocyte plasma membrane of the rainbow trout, Salmo gairdneri: Associated immunoglobulin and heteroantigens
Series title:
Comparative Biochemistry and Physiology, Part B: Comparative Biochemistry
DOI:
10.1016/0305-0491(83)90285-7
Volume:
76
Issue:
3
Year Published:
1983
Language:
English
Publisher:
Elsevier
Contributing office(s):
Leetown Science Center
Description:
7 p.
First page:
515
Last page:
521