Mycoplasma contamination in fish cell lines: An evaluation of detection by differential incorporation of 3H-uridine and 14C-uracil
Journal of Fish Diseases
By: C. L. Schultz, B. C. Lidgerding, P. E. McAllister, and F. M. Hetrick
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Abstract
Differential incorporation of uridine and uracil was used to assay for mycoplasma contamination in five fish cell lines: bluegill fry (BF-2), chinook salmon embryo (CHSE-214), epithelioma papillosum cyprini (EPC), fathead minnow (FHM) and rainbow trout gonad (RTG-2). The method was not suitable for monitoring BF-2, CHSE-214, FHM, and RTG-2 cell lines because they incorporated uracil. Differential incorporation of uridine and uracil may be applicable for screening EPC cells because only this cell line could distinguish cultures experimentally infected with Mycoplasma orale from cultures known to be free from microbial contaminants.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Mycoplasma contamination in fish cell lines: An evaluation of detection by differential incorporation of 3H-uridine and 14C-uracil |
| Series title | Journal of Fish Diseases |
| DOI | 10.1111/j.1365-2761.1986.tb00990.x |
| Volume | 9 |
| Issue | 2 |
| Year Published | 1986 |
| Language | English |
| Publisher | Wiley |
| Contributing office(s) | Leetown Science Center |
| Description | 6 p. |
| First page | 117 |
| Last page | 122 |
| Google Analytic Metrics | Metrics page |