PCR-based assays for the fish pathogen Aeromonas salmonicida: Further evaluation and validation of three PCR primer sets with infected fish
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Abstract
Two Aeromonas salmonicida-specific polymerase chain reaction (PCR) tests and 1 A. salmonicida subsp. salmonicida-specific PCR test were used to screen salmonid populations that were either overtly or covertly infected with A. salmonicida subsp. salmonicida. It was demonstrated that these PCR assays could be used to replace the biochemical testing currently employed to confirm the identity of A. salmonicida isolates cultured from infected fish. The AP and PAAS PCR assays were also capable of direct detection of A. salmonicida in overtly infected fish, with mucus, gill and kidney samples most likely to yield a positive result. Culture was a more reliable method for the direct detection of A. salmonicida in covertly infected salmonids than was the direct PCR testing of tissue samples, with the AP and PAAS PCRs having a lower detection limit (LDL) of approximately 4 x 105 colony-forming units (CFU) g-1 sample.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | PCR-based assays for the fish pathogen Aeromonas salmonicida: Further evaluation and validation of three PCR primer sets with infected fish |
| Series title | Diseases of Aquatic Organisms |
| DOI | 10.3354/dao049139 |
| Volume | 49 |
| Issue | 2 |
| Year Published | 2001 |
| Language | English |
| Publisher | Inter-Research |
| Contributing office(s) | Leetown Science Center |
| Description | 6 p. |
| First page | 139 |
| Last page | 144 |
| Google Analytic Metrics | Metrics page |