Gill nets and trawls were fished by the Bureau of Commercial Fisheries R/V Cisco during September 19-23, 1964, at several locations and depths in the offshore United States waters of Lake Ontario. Water temperatures were low (3.7-8.3 A?C) at all fishing stations except one (16.4 A?C). Supplementary data were provided by the Bureau's R/V Kaho in 1966. Alewives and smelt were common. Ciscoes were extremely scarce, but large; most of those caught were bloaters. Slimy sculpins were abundant, but no deepwater sculpins were caught. Yellow perch were scarce. Although the warm water species were inadequately sampled, trout-perch seemed to be abundant. Other species, all caught in small numbers, were lake trout, spottail shiners, burbot, threespine sticklebacks, and johnny darters from cold water and northern pike, lake chubs, white suckers, white bass, white perch, and rock bass from warm water.