Formalin preservation of avian blood for organochlorine analysis

By:  and 
Edited by: D.W. Lamb and E.E. Kenaga

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Abstract

Blood biopsy for chemical analysis is a valuable technique for evaluating chemical exposure of birds in the wild without harming the birds. Field conditions, however, often make sample storage difficult. Better methods than freezing are needed to improve the interpretive value of chemical analysis of the sample. The use of formalin was explored for this purpose. A pooled sample of blood containing naturally incorporated 1,1-bis-(p-chlorophenyl)-2,2,2-trichloroethane (DDT), 2,2-bis-(p-chlorophenyl)1,1 dichloroethylene (DDE), and dieldrin was subdivided into 30 samples, of which 10 were frozen, 10 more were kept at room temperature, and 10 were formalinized by adding I part of chemically pure formalin to 20 parts of blood. The formalinized samples yielded the highest and least variable concentrations of chemicals. The field procedures are outlined.
Publication type Book chapter
Publication Subtype Book Chapter
Title Formalin preservation of avian blood for organochlorine analysis
Series number 757
DOI 10.1520/STP28374S
Year Published 1981
Language English
Publisher American Society for Testing and Materials
Publisher location Philadelphia, Pennsylvania
Contributing office(s) Patuxent Wildlife Research Center
Description 164
Larger Work Type Book
Larger Work Subtype Other Government Series
Larger Work Title Avian and Mammalian Wildlife Toxicology: Second Conference
First page 93
Last page 97
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